Device

Part:BBa_K1365100:Design

Designed by: Rick Elbert   Group: iGEM14_Groningen   (2014-10-08)


anti Staphylococcus aureus Secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1168
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RBS was assembled to BBa_K1365111 and BBa_I746104 with PCR. afterwhich all the parts were assembled with gibson assembly.


Source

the P2 promotor and Double terminator came from the iGEM distribution kit 2014. NisA originates from the genomic sequence of L. lactis NZ9700. DspB was synthesized by IDT.

References